Drosophila Hemi-Thorax Staining and Imaging

Drosophila hemi-thoraces were isolated and stained as described above with phalloidin Alexa488 (1:2000) and the two nanobodies coupled to either P1, P3, or PS3 oligos (about 50 nM) overnight. Before embedding the samples into the chamber, they were washed two times with PBS + 1% Triton. Hemi-thoraces were embedded as described above. Before assembling the chamber, the cover slip was treated with 90 nm diameter gold nanoparticles (cat#: G-90-100, Cytodiagnostics, 1:10 dilution into methanol). After assembling, the chamber was filled with imaging buffer containing the complementary P1, P3, or PS3 imaging oligos (see below for imaging conditions) and sealed with Picodent glue.