Investigating STAT3 Phosphorylation in G-CSF Signaling

OVA_257–264 (SIINFEKL) was obtained from AnaSpec (Fremont, CA). Antibodies (Abs) against phospho-STAT3 (Tyr705) and STAT3 were from Cell Signaling Technology (Beverly, MA), and Ab against GAPDH was from Abcam (Cambridge, MA). Neutralizing Ab to G-CSF (MAB414) and isotype control (IgG1) were from R&D Systems (San Diego, CA). Neutralizing Ab to Gr-1 (RB6-8C5) and isotype control (IgG2b) were from BioXcell (West Lebanon, NH). Recombinant murine G-CSF and GM-CSF were from R&D Systems (San Diego, CA). Lentiviral expression of SOCS3 was generated as described (29 (link)).

Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link: Access Free Full Text.

Yu H., Liu Y., McFarland B.C., Deshane J.S., Hurst D.R., Ponnazhagan S., Benveniste E.N, & Qin H. (2015). SOCS3 Deficiency in Myeloid Cells Promotes Tumor Development: Involvement of STAT3 Activation and Myeloid-Derived Suppressor Cells. Cancer immunology research, 3(7), 727-740.

Publication 2015

OVA257–264 (SIINFEKL) Ab against GAPDH isotype control (IgG1) GM-CSF

Antibodies G csf Gapdh Gm csf Igg1 Igg2b Isotype Murine Stat3

Corresponding Organization : University of Alabama at Birmingham

Top 5 similar protocols

Variable analysis

independent variables

OVA_257–264 (SIINFEKL) obtained from AnaSpec (Fremont, CA)
Recombinant murine G-CSF and GM-CSF obtained from R&D Systems (San Diego, CA)
Lentiviral expression of SOCS3 generated as described (29 (link))

dependent variables

Not explicitly mentioned

control variables

Antibodies (Abs) against phospho-STAT3 (Tyr705) and STAT3 from Cell Signaling Technology (Beverly, MA)
Ab against GAPDH from Abcam (Cambridge, MA)
Neutralizing Ab to G-CSF (MAB414) and isotype control (IgG1) from R&D Systems (San Diego, CA)
Neutralizing Ab to Gr-1 (RB6-8C5) and isotype control (IgG2b) from BioXcell (West Lebanon, NH)

controls

Positive control: Antibodies (Abs) against phospho-STAT3 (Tyr705) and STAT3
Negative controls: Isotype control (IgG1) for neutralizing Ab to G-CSF, and isotype control (IgG2b) for neutralizing Ab to Gr-1

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!