OVA257–264 (SIINFEKL) was obtained from AnaSpec (Fremont, CA). Antibodies (Abs) against phospho-STAT3 (Tyr705) and STAT3 were from Cell Signaling Technology (Beverly, MA), and Ab against GAPDH was from Abcam (Cambridge, MA). Neutralizing Ab to G-CSF (MAB414) and isotype control (IgG1) were from R&D Systems (San Diego, CA). Neutralizing Ab to Gr-1 (RB6-8C5) and isotype control (IgG2b) were from BioXcell (West Lebanon, NH). Recombinant murine G-CSF and GM-CSF were from R&D Systems (San Diego, CA). Lentiviral expression of SOCS3 was generated as described (29 (link)).