GC Analysis of Acidolysis Products

Gas chromatography (GC) was applied for analysis of acid profile of acidolysis products (PC-egg, LPC-egg, 3-OMe-CA-PC, 3-OMe-CA-LPC) and standards (PC-egg, 3-OMe-CA). For this purpose purified samples (SPE/column chromatography methods) were derivatized into corresponding to them methyl esters as described before [22 (link)] and analyzed on an Agilent 6890N [20 (link)]. Retention times of products were compared with retention time of a standard (Supelco 37 FAME Mix, Sigma Aldrich).

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Okulus M., Rychlicka M, & Gliszczyńska A. (2021). Enzymatic Production of Biologically Active 3-Methoxycinnamoylated Lysophosphatidylcholine via Regioselctive Lipase-Catalyzed Acidolysis. Foods, 11(1), 7.

Publication 2021

Supelco 37 FAME Mix

Acid Chromatography Esters Gas chromatography Retention

Corresponding Organization : Wroclaw University of Environmental and Life Sciences

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Variable analysis

independent variables

Acid profile of acidolysis products (PC-egg, LPC-egg, 3-OMe-CA-PC, 3-OMe-CA-LPC) and standards (PC-egg, 3-OMe-CA)

dependent variables

Retention times of products

control variables

Purified samples (SPE/column chromatography methods) were derivatized into corresponding to them methyl esters
Analyzed on an Agilent 6890N
Retention times of products were compared with retention time of a standard (Supelco 37 FAME Mix, Sigma Aldrich)

positive controls

Supelco 37 FAME Mix (Sigma Aldrich)

negative controls

Not explicitly mentioned

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