The AT980 ΔdapD strain requiring mDAP for growth was earlier described [26 (link)]⁠. The MLD2502 strain derived from BW25113 that carries a deletion of the chromosomal mpl gene (Δmpl::CmR) was described previously [60 (link)]. The ΔdapDΔmpl double mutant strain used in this work was constructed by transduction of the Δmpl::CmR mutation into the AT980 strain by phage P1 transduction [61 ]⁠.
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