Antimicrobial Susceptibility Testing of MNPECs

Antimicrobial susceptibility testing (AST) was performed by broth microdilution method as our previously described (Liu et al., 2021b (link)). Briefly, 0.5 McFarland inoculum suspensions were diluted 1:100 to a final inoculum density of 10⁶ CFU/ml. Ampicillin (AMP), ceftazidime (CAZ), cefotaxime (CTX), aztreonam (AZM), tetracycline (TET), doxycycline (DOX), ciprofloxacin (CIP), enrofloxacin (ENR), gentamicin (GEN), sulfamethoxazole (SMZ), fosfomycin (FOS), florfenicol (FFC), colistin (COL), and meropenem (MEM) (Solarbio, Beijing, China) were selected to determine the minimum inhibitory concentrations (MICs) of MNPECs. The MICs were recorded as the lowest concentrations of antimicrobials in the wells, where no visible bacteria growth was observed. Escherichia coli ATCC 25922 was used as standard reference strains.

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Xiang Y., Liu Z., Yu G., Song Y., Li Y., Geng X., Ma L., Guo J., Tan L, & Chen P. (2023). Genetic characteristic of coexisting of mcr-1 and blaNDM-5 in Escherichia coli isolates from lesion-bearing animal organs. Frontiers in Microbiology, 14, 1116413.

Publication 2023

gentamicin florfenicol colistin meropenem (MEM)

Ampicillin Antimicrobials Aztreonam Bacteria Cefotaxime Ceftazidime Ciprofloxacin Colistin Doxycycline Enrofloxacin Escherichia coli Florfenicol Fosfomycin Gentamicin Meropenem Minimum inhibitory concentrations Strains Sulfamethoxazole Susceptibility Tetracycline

Corresponding Organization : Second Affiliated Hospital of Zhengzhou University

Other organizations : Shenzhen Technology University

Top 5 similar protocols

Variable analysis

independent variables

Ampicillin (AMP)
Ceftazidime (CAZ)
Cefotaxime (CTX)
Aztreonam (AZM)
Tetracycline (TET)
Doxycycline (DOX)
Ciprofloxacin (CIP)
Enrofloxacin (ENR)
Gentamicin (GEN)
Sulfamethoxazole (SMZ)
Fosfomycin (FOS)
Florfenicol (FFC)
Colistin (COL)
Meropenem (MEM)

dependent variables

Minimum Inhibitory Concentrations (MICs)

control variables

Inoculum density (10^6 CFU/ml)
Escherichia coli ATCC 25922 as standard reference strain

controls

Escherichia coli ATCC 25922 as standard reference strain

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