Monoclonal antibodies recognizing human OPN were produced as previously described [20 (link)]. Antibody 9–3 was raised against peptide C-GDSVVYG while antibody 21–5 was raised against peptide C-TYDGRGDSVVYG-CO-NH2.
An IgG1 isotype control was used (M5284, Sigma) for in vitro experiments.
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