ℒ-fucose (Sigma-Aldrich, F2252-5G) was used to reconstitute fucosylation in Slc35c1-deficient cells. Inhibition of fucosylation in HDF, HL-60, MDCK and VERO cells was achieved using 2F-Peracetyl-Fucose (Merck Chemicals and Life Science, 344827)22 (link); murine organoids were treated with 2-deoxy-2-fluoro-ℒ-Fucose (Carbosynth, MD06089)23 (link). Sialylation was inhibited using 3Fax-Peracetyl Neu5Ac (Merck Chemicals and Life Science, US1566224)22 (link).
Other organizations :
Institute of Molecular Biotechnology, Austrian Academy of Sciences, Zentrum für Kinderheilkunde, Heidelberg University, University Hospital Heidelberg, Norwegian Cancer Society, Oslo University Hospital, Scripps Research Institute, Research Institute of Molecular Pathology, Vienna Biocenter, University Hospital Münster
2F-Peracetyl-Fucose (Merck Chemicals and Life Science, 344827)
2-deoxy-2-fluoro-ℒ-Fucose (Carbosynth, MD06089)
3Fax-Peracetyl Neu5Ac (Merck Chemicals and Life Science, US1566224)
dependent variables
Fucosylation in Slc35c1-deficient cells
Inhibition of fucosylation in HDF, HL-60, MDCK and VERO cells
Inhibition of sialylation
control variables
Not explicitly mentioned
positive controls
Not specified
negative controls
Not specified
Annotations
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