pCS2+_CBE4max-SpG plasmid has been used to generate CBE4max-SpG mRNA in vitro. pCS2+_CBE4max-SpRY plasmid has been used to generate CBE4max-SpRY mRNA in vitro. Each plasmid was linearized with NotI restriction enzyme and mRNAs were synthesized by in vitro transcription with 1 µL of GTP from the kit added to the mix and lithium chloride precipitation (using the mMESSAGE mMACHINE sp6 Ultra kit #AM1340, Ambion).
pCMV_ancBE4max8 (link) (a gift from David Liu _ Addgene plasmid #112094) has been linearized using AvrII restriction enzyme; mRNAs were synthesized by in vitro transcription with 1 µL of GTP from the kit added to the mix and lithium chloride precipitation (using the mMESSAGE mMACHINE T7 Ultra kit #AM1345, Ambion).
pCMV_ancBE4max8 (link) (a gift from David Liu _ Addgene plasmid #112094) has been linearized using AvrII restriction enzyme; mRNAs were synthesized by in vitro transcription with 1 µL of GTP from the kit added to the mix and lithium chloride precipitation (using the mMESSAGE mMACHINE T7 Ultra kit #AM1345, Ambion).
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