Analysis of NF-κB Inhibition and Golgi Markers

NF-κB inhibitor BAY 11–7085 (SC-202490, Santa Cruz Biotechnology, Dallas, TX, USA) and monensin (M5273, Sigma Chemical Co., St. Louis, MO, USA) were dissolved in dimethyl sulfoxide (DMSO) and diluted in fresh medium before use. Antibodies used in this study and their sources are as follows: anti-golgin-97 (ab84340; Abcam Inc., Cambridge, MA, USA); anti-E-cadherin (sc-7870), anti-p65 (sc-8008), anti-GAPDH (sc-6215), and anti-Lamin A/C (sc-6215) (Santa Cruz Biotechnology, Dallas, TX, USA); anti-IκB (#4814; Cell Signaling Technology, Beverly, MA, USA); anti-phospho-NF-κB p65 (Ser529) (GTX38622; GeneTex, Irvine, CA, USA); anti-β-actin (Millipore, Bedford, MA, USA); anti-TGN46 (AD Serotec, AHP500; Bio-Rad Laboratories, Inc., Hercules, CA, USA); and anti-GM130 (610823; BD Transduction Laboratories, Lexington, KY, USA). The rabbit polyclonal antibody against human Arl1 was produced in-house as described previously [28 (link)]. Anti-GCC185 was kindly provided by Dr. Fang-Jen S. Lee, National Taiwan University, Taiwan.