Cell Culture Conditions for Diverse Cell Lines

Huh7.5 (cancerous Human hepatocytes, RRID:CVCL_7927), HEK293T (transformed human embryonic kidney cells RRID:CVCL_0063), A549, NIH-3T3 (spontaneously immortalized NIH Swiss mouse embryonic fibroblasts, RRID:CVCL_0594), MCA-RH 7777 (cancerous buffalo rat hepatocytes, RRID:CVCL_0444), FEA (spontaneously immortalized cat embryonic fibroblasts, RRID:CVCL_UG17), CRFK (spontaneously immortalized cat kidney epithelial cells, RRID:CVCL_2426), and Vero (spontaneously immortalized green monkey kidney epithelium, RRID:CVCL_0059) cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM; Gibco #41965–039) supplemented with 10% heat-inactivated fetal calf serum (Sigma-Aldrich #F75424), and 100 μg/mL of normocin (InvivoGen #ant-nr-2) and grown in cell culture flasks at 37°C in a humidified incubator containing 5% CO2. I/1Ki (boa constrictor kidney cells)[83 (link)] and I/1Ki-Δ (referred to as I/1Ki-SDeV in this paper) [13 (link)] were maintained in Minimal Essential Medium Eagle (MEM; Gibco #31095–029) supplemented with 20% heat-inactivated fetal calf serum (Sigma-Aldrich #F75424), and 100 μg/mL of normocin (InvivoGen #ant-nr-2), and grown in cell culture flasks at 30°C in a humidified incubator containing 5% CO2. All cell lines used in the study were mycoplasma-free and were checked regularly for mycoplasma contamination.

Free full text: Click here

Khalfi P., Denis Z., McKellar J., Merolla G., Chavey C., Ursic-Bedoya J., Soppa L., Szirovicza L., Hetzel U., Dufourt J., Leyrat C., Goldmann N., Goto K., Verrier E., Baumert T.F., Glebe D., Courgnaud V., Gregoire D., Hepojoki J, & Majzoub K. (2024). Comparative analysis of human, rodent and snake deltavirus replication. PLOS Pathogens, 20(3), e1012060.

Publication 2024

DMEM F75424 ant-nr-2

Corresponding Organization : Université de Strasbourg

Top 5 similar protocols

Variable analysis

independent variables

Cell lines used: Huh7.5, HEK293T, A549, NIH-3T3, MCA-RH 7777, FEA, CRFK, Vero, I/1Ki, I/1Ki-Δ

dependent variables

Cell growth and maintenance

control variables

Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% heat-inactivated fetal calf serum and 100 μg/mL of normocin for Huh7.5, HEK293T, A549, NIH-3T3, MCA-RH 7777, FEA, CRFK, and Vero cells
Minimal Essential Medium Eagle (MEM) supplemented with 20% heat-inactivated fetal calf serum and 100 μg/mL of normocin for I/1Ki and I/1Ki-Δ cells
Incubator conditions: 37°C, 5% CO2 for Huh7.5, HEK293T, A549, NIH-3T3, MCA-RH 7777, FEA, CRFK, and Vero cells; 30°C, 5% CO2 for I/1Ki and I/1Ki-Δ cells

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!