To demonstrate the potential clinical application of contrast-free quantitative ultrasound microvasculature imaging, we performed the technique on a group of patients with suspicious breast lesions or thyroid nodules. Before quantifying vessel morphological features from ultrasound microvasculature images, one must perform multiple preprocessing steps. The first step is image formation, which reconstructs the microvasculature image from a sequence of plane wave ultrasound images [20 (link)]. Second, vessel filtering is used to enhance the structure of vessels and provide adequate background separation for segmentation. Morphological filtering, vessel segmentation, and skeletonization occur last. The main contribution of this paper is in the use of spectral filtering, vessel segmentation, filtering and vessel quantification.