Fluorescent Protein Constructs for Cellular Imaging

Expression constructs encoding for human flotillin-1 or flotillin-2 were a gift from V. Niggli (University of Bern). TCRζ-PSCFP2 was provided by Prof. K. Gaus (University of New South Wales). PA-mCherry expression backbone was obtained from Clontech. TCRζ PA-mCherry was made as described in [15 (link)]. mCherry-Rab5 was a gift from Gia Voeltz (Addgene plasmid # 49,201). mCherry-Rab11a was a gift from Michael Davidson (Addgene plasmid # 55,124). pBGPa-CMV-GFP-OSBP PH domain was a gift from Tim Levine (Addgene plasmid # 58,724). LACT-C2 and 2xFYVE expression constructs were a gift from Prof. Rob Parton (University of Queensland).