Tissues (5 μm) were de-paraffinized in xylene and rehydrated with ethanol before antigen retrieval. After antigen retrieval in EDTA solution, the sections were washed with PBS for 15 min and treated with blocking buffer for 30 min at room temperature. For Lamin B1 staining, sections were incubated with anti-rabbit Lamin B1 antibody (1:200; AF5161-50; Affinity Biosciences; Melbourne, Australia) overnight at 4°C, and the fluorescently labeled secondary antibody (1:1000; coralite594, SA00013-4; Proteintech; Wuhan, Hubei, China) for 1 h at room temperature. For Lamin B1 and Fibronectin staining in cells, cells were seeded on glass slides and the slides were fixed in 4% formaldehyde in PBS for 15 min, washed three times in PBS, and then permeabilized with 0.1% TritonX-100 in PBS for 10 min. Slides were then blocked with 3% BSA in PBS for 1 h at room temperature followed by incubation with the primary antibody (Lamin B1, 1:200; Fibronectin, 1:200, 15613–1-AP, Proteintech) at 4°C overnight and the secondary antibody for 1 h at room temperature. Nuclei were stained with DAPI (HNFD-02; HelixGen). Images were taken with Olympus BX53 microscope.