Downregulating AnxA6 in Breast Cancer Cells

Small-hairpin RNAs (shRNAs) targeting the coding sequence of AnxA6 (A6sh) in pGIPZ lentiviral vector, a non-silencing shRNA control (26 (link)) (GE Dharmacon), were used to prepare lentiviral stocks in 293 T cells, and the viral particles were used to infect LAP-R (P3) breast cancer cells as described previously (25 (link)). The resulting subclones of LAP-R cells were denoted LAP-R-NSC, LAP-R-A6sh5 and LAP-R-A6sh2. Downregulation of AnxA6 was verified by immunoblotting.

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Widatalla S.E., Korolkova O.Y., Whalen D.S., Goodwin J.S., Williams K.P., Ochieng J, & Sakwe A.M. (2018). Lapatinib-induced annexin A6 upregulation as an adaptive response of triple-negative breast cancer cells to EGFR tyrosine kinase inhibitors. Carcinogenesis, 40(8), 998-1009.

Publication 2018

pGIPZ lentiviral vector

293 t cells Breast cancer Coding sequence Downregulation Shrna Vector Viral particles

Corresponding Organization : Meharry Medical College

Other organizations : North Carolina Central University

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Variable analysis

independent variables

Small-hairpin RNAs (shRNAs) targeting the coding sequence of AnxA6 (A6sh) in pGIPZ lentiviral vector
A non-silencing shRNA control (26)

dependent variables

Downregulation of AnxA6 (verified by immunoblotting)

control variables

The viral particles were used to infect LAP-R (P3) breast cancer cells

controls

A non-silencing shRNA control (26)

Annotations

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