Antifungal Potential of Naringin

This study was conducted on one candidate biomarker (naringin) identified in Seville oranges (the tolerant cultivar). A 20 000 mg/L stock solution of naringin was prepared and the in vitro exposure to 1 000, 3 000, 4 000, 5 000, 7 500 and 10 000 mg/L concentrations of naringin was used to evaluate the antifungal properties of the candidate biomarker. In addition, three fungicides, Demildex, MHTCO₃ and cuprous oxide, were tested at 2000 mg/L, 10 000 mg/L and 18 000 mg/L respectively and served as positive controls while water was used as negative control. The CSA media and inoculation were performed as mentioned in Sect. 3.9.1. For each compound, 10 replicates of each concentration tested were prepared. After 14 days of incubation at 27 °C ± 2 °C, mycelial growth was measured (in mm) with a digital calliper (Absolute Digimatic-Mitutoyo Corp. Japan). Percentage inhibition of mycelial growth was determined as described by Plaza et al. (2004 (link)).