Excisional Skin Wound Healing in Mice

A single full-thickness excisional skin wound was made on the shaved flank skin of mice using a 4 mm-diameter biopsy punch (Kai Industries, Japan). Wounds were imaged using a Nikon COOLPIX B500 digital camera each day and wound size measured using calipers¹⁹ on a daily basis for up to nine days post injury. Wound area was calculated as described¹⁹ and presented as the percentage of initial wound size.^{10 (link)} In a second set of experiments, anti-podoplanin antibody (clone 8.1.1, 100 μg)^{13 (link),17 (link),20 (link)} or IgG isotype control were intravenously injected 24 hours (h) before and again 24 h after wounding. Wound size was monitored for three days post injury.

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Wichaiyo S., Lax S., Montague S.J., Li Z., Grygielska B., Pike J.A., Haining E.J., Brill A., Watson S.P, & Rayes J. (2019). Platelet glycoprotein VI and C-type lectin-like receptor 2 deficiency accelerates wound healing by impairing vascular integrity in mice. Haematologica, 104(8), 1648-1660.

Publication 2019

COOLPIX B500

Anti antibody Biopsy Camera Clone Injury Isotype Mice Skin Wound

Corresponding Organization :

Other organizations : Mahidol University, University of Birmingham, Sechenov University

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Variable analysis

independent variables

Injection of anti-podoplanin antibody (clone 8.1.1, 100 μg) or IgG isotype control

dependent variables

Wound size (measured using calipers and calculated as percentage of initial wound size)

control variables

Shaved flank skin of mice
Wound size measurement using Nikon COOLPIX B500 digital camera and calipers

controls

Positive control: IgG isotype control
Negative control: Not explicitly mentioned

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