hESC (WA01/H1 line; WiCell) were cultured on Matrigel-coated plates (BD Biosciences) in Essential 8 medium (A1517001; Life Technologies). Differentiation was carried out following a seven-stage protocol as previously described (21 (link)–23 (link)) with further modifications. The glucose concentration of each stage was as follows: S1–S4 cells were cultured in 10.5 mmol/L glucose; S5–S6 cells were cultured in 20.5 mmol/L glucose; and S7 cells were cultured in 5.5 mmol/L glucose.
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Stepwise hESC Differentiation
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Corresponding Organization : University of Exeter
Other organizations : Centre for Genomic Regulation, Dokuz Eylül University, Hamad Medical Corporation, Weill Cornell Medical College in Qatar, Royal Devon & Exeter NHS Foundation Trust
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Variable analysis
independent variables
- Glucose concentration during different stages of differentiation
- Outcomes of the 7-stage differentiation protocol
- Culture of hESC (WA01/H1 line; WiCell) on Matrigel-coated plates
- Use of Essential 8 medium
- No positive or negative controls were explicitly mentioned in the provided information.
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