Balb/c mice were injected with 1x106 CT26 cells in the shoulder. Starting on day 7 post-inoculation when the tumors have an average tumor diameter of about 3–4 mm, mice were injected i.p. with 12.5 mg/kg of either anti-CD137 antibody (clone 3H3; BioXCell) or anti-PD-L1 (clone 10F.9G2; BioXCell) every other day for four treatments. On day 15 post-tumor inoculation, mice were injected with 89Zr-radiolabeled malDFO-169 cDb for immuno-PET imaging and biodistribution the following day (22 h post-injection). Average tumor diameter was calculated using calipers on days 7, 11 and 15 post-tumor inoculation.
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Immunotherapy and Tumor Imaging in Mice
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Corresponding Organization : University of California, Los Angeles
Other organizations : Broad Center, Howard Hughes Medical Institute, Institute for Molecular Medicine
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Variable analysis
independent variables
- Anti-CD137 antibody (clone 3H3; BioXCell)
- Anti-PD-L1 (clone 10F.9G2; BioXCell)
- Tumor diameter (measured on days 7, 11 and 15 post-tumor inoculation)
- Biodistribution of 89Zr-radiolabeled malDFO-169 cDb (measured on day 16 post-tumor inoculation)
- Balb/c mice
- CT26 cells (1x10^6 cells injected in the shoulder)
- Timing of antibody treatments (every other day for four treatments, starting on day 7 post-inoculation)
- None specified
- None specified
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