Blood Sample Preparation and Analysis

The blood samples (5 mL) were collected by using Vacutainer K2E-EDTA tubes and transferred into 15 mL centrifuge tube contained Ficoll-paque PLUS medium [hall blood/Ficoll-paque PLUS medium = 4/3 (v/v)] (GE Healthcare Life Sciences, Pittsburgh, PA, USA), then centrifuged (3000 rpm, 30 min). After centrifugation, the supernatant was collected in sterile tubes and 1 N of acetic acid was added to prevent degradation. All the samples were stored at −80 °C and the storage time was less than one year. The plasma levels of fasting blood glucose (Glu-AC) and lipid profiles (TG, TCH, HDL-C, and LDL-C) were determined using the COBAS Integra 800 autoanalyzer (Roche Diagnostics, Basel, Switzerland) [25 (link),26 (link)].

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Dongoran R.A., Lin T.J., Byekyet A., Tang S.C., Yang J.H, & Liu C.H. (2020). Determination of Major Endogenous FAHFAs in Healthy Human Circulation: The Correlations with Several Circulating Cardiovascular-Related Biomarkers and Anti-Inflammatory Effects on RAW 264.7 Cells. Biomolecules, 10(12), 1689.

Publication 2020

Ficoll-paque PLUS medium COBAS Integra 800 autoanalyzer

Acetic acid Blood Blood glucose Centrifugation Diagnostics Edta Ficoll Glucose Lipid Plasma Sterile

Corresponding Organization : Tzu Chi University

Other organizations : National Taichung University of Science and Technology, Changhua Christian Hospital

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Variable analysis

independent variables

Blood/Ficoll-paque PLUS medium ratio (4/3 (v/v))

dependent variables

Fasting blood glucose (Glu-AC)
Lipid profiles (TG, TCH, HDL-C, and LDL-C)

control variables

Blood sample volume (5 mL)
Storage time (less than one year)
Storage temperature (-80 °C)
Assay method (COBAS Integra 800 autoanalyzer)

controls

No positive or negative controls were explicitly mentioned in the input protocol.

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