Cell-surface CD expressions were detected by flow cytometry using a BD FACSCalibur (BD Biosciences, Haryana, India), as described previously [71 (link)]. An FITC-conjugated anti-mouse CD3 antibody (Cat# 100203, BioLegend, San Diego, CA, USA), FITC-conjugated anti-mouse CD4 antibody (Cat# 116003, BioLegend) and PE-conjugated anti-mouse CD25 antibody (Cat# 101904, BioLegend) were used.
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