All animal procedures were approved by an institutional animal care committee. To model osteogenesis imperfecta, oim on a C57Bl/6 background (obtained from Dr. Charlotte Philips, Univ. of Missouri) that lack functional Col1a2 were utilized [27 (link)]. OIM homozygous mice were used for all experiments. OIM mice were genotyped as previously described [28 (link)]. Donor cells were sourced from mice that are a combination of the three previously described lines: αSMACreERT2 [24 (link)], Col2.3GFP [26 (link)] and Ai9 reporter mice (stock # 007909, Jackson Laboratory) [29 (link)]. To generate αSMACreERT2/Ai9/Col2.3GFP mice, αSMACreERT2/Col2.3GFP were bred with Ai9, and termed SMA9/Col2.3GFP. To assess recombination efficiency αSMAGFP mice were crossed with αSMACreERT2/Ai9 [30 (link)].
Protocol detail
Modeling Osteogenesis Imperfecta in Mice
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Animal
Animal care committee
Cells
Col1a2
Donor
Homozygous
Mice
Osteogenesis imperfecta
Recombination
Corresponding Organization : UConn Health
Other organizations : University of Auckland
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Variable analysis
independent variables
- Genotype: oim (Col1a2 deficient) on C57Bl/6 background
- Not explicitly mentioned
- Mouse strain: C57Bl/6 background
- Genotyping: OIM homozygous mice used for all experiments
- Positive control: Not specified
- Negative control: Not specified
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