Two different types of compounds (Table S-6 ) were used as internal standards. Type I internal standards were added to samples before extraction to mimic the extraction of prostaglandins, diols, epoxides and other oxylipins. The type I internal standards include 6-keto-PGF1α-d4, PGE2-d4, 10,11 DHHep, 20 HETE-d6, 9 (S) HODE-d4, 5 HETE-d8, 11,12 EET-d8 and non-endogenous odd chain length monounsaturated fatty acids, 10,11 dihydroxynondecanoic acid 10,11-DHN. Type II internal standard was added at the last step before analysis to account for changes in volume and instrument variability. A synthetic acid, 1-cyclohexyl-dodecanoic acid urea (CUDA), was selected as type II internal standard.
The analytes were linked to their corresponding Type I internal standards for the purpose of quantification.
The analytes were linked to their corresponding Type I internal standards for the purpose of quantification.
