Whole-mount Oil Red O stain was performed as described (Kim et al., 2015 (link)). For histologic analysis, larvae were fixed in 10% formalin and embedded in paraffin. 6 μm sections were stained with hematoxylin and eosin (H&E). Images were obtained on a Carl Zeiss Discovery V8 stereomicroscope using a Zeiss Axioncam MRc5 camera (Carl Zeiss, Jena, Germany).
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