IL-23R Expression in K562 Cells

Among the human myeloid and lymphoid cell lines, the mRNA expression of IL-23R is more abundant in K562 cells than in Jurkat cells [12 (link)]. We chose K562 cells for this study. K562 cells, a human erythroleukemia line, purchased from the American Type Culture Collection (Manassas, VA, USA) were cultured in medium with or without the presence of IL-23 (20 ng/ml; Sigma-Aldrich, St. Louis, MO, USA) for 3 days according to previous studies [13 (link), 14 (link)] with some modifications. These cells were used for subsequent analysis.

Free full text: Click here

Lai N.S., Yu H.C., Tung C.H., Huang K.Y., Huang H.B, & Lu M.C. (2018). Aberrant expression of interleukin-23-regulated miRNAs in T cells from patients with ankylosing spondylitis. Arthritis Research & Therapy, 20, 259.

Publication 2018

K562 cells IL-23

Cell lines Cells Erythroleukemia Human Il 23r Jurkat cells K562 cells Lymphoid Mrna

Corresponding Organization :

Other organizations : Tzu Chi Foundation, National Chung Cheng University, Tzu Chi University

Top 5 similar protocols

Variable analysis

independent variables

Presence of IL-23 (20 ng/ml)

dependent variables

MRNA expression of IL-23R

control variables

K562 cells, a human erythroleukemia line
Culture medium

controls

K562 cells cultured in medium without the presence of IL-23 (negative control)

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!