Immunohistochemical staining for MMR proteins was performed, using a primary antibody to MLH1 (clone G168–15; Biocare Medical, Concord, CA), MSH2 (clone FE11; Biocare Medical), MSH6 (clone BC/44; Biocare Medical), or PMS2 (clone A16–4; Biocare Medical), and a polymer detection system (Dako, Carpinteria, CA) on an automated staining system (Dako), on the sections (5 μm thick) from the prostate TMA, as described previously.[16 (link)] All stains were quantified independently by 2 pathologists (MS and HM) who were blinded to sample identity. Convincing nuclear staining of each protein in at least 1% of tumor cells was considered to be positive. Cases with discrepancies in the positivity were re-reviewed simultaneously by the 2 pathologists until a consensus was reached.
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Immunohistochemical Evaluation of MMR Proteins
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Other organizations : University of Rochester Medical Center
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Variable analysis
independent variables
- Primary antibody to MLH1 (clone G168–15; Biocare Medical, Concord, CA)
- Primary antibody to MSH2 (clone FE11; Biocare Medical)
- Primary antibody to MSH6 (clone BC/44; Biocare Medical)
- Primary antibody to PMS2 (clone A16–4; Biocare Medical)
- Nuclear staining of each protein (MLH1, MSH2, MSH6, PMS2) in at least 1% of tumor cells
- Sections (5 μm thick) from the prostate TMA
- Polymer detection system (Dako, Carpinteria, CA) on an automated staining system (Dako)
- Quantification of stains independently by 2 pathologists (MS and HM) who were blinded to sample identity
- Cases with discrepancies in the positivity were re-reviewed simultaneously by the 2 pathologists until a consensus was reached
- Positive control: Not explicitly mentioned
- Negative control: Not explicitly mentioned
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