Cytotoxicity Evaluation by LDH Assay

Cell viability was monitored with the lactate dehydrogenase (LDH) release assay using the CytoTox96^® non-radioactive cytotoxicity kit (Promega, Stockholm, Sweden), as previously described [16 (link)]. The samples were analyzed using the Tecan Infinite^® F200 plate reader operating with Magellan v7.2 software (Männedorf, Switzerland). The percentage of cell viability was calculated based on the ratio between the absorbance of each sample and the negative control sample.

Free full text: Click here

Keshavan S., Andón F.T., Gallud A., Chen W., Reinert K., Tran L, & Fadeel B. (2021). Profiling of Sub-Lethal in Vitro Effects of Multi-Walled Carbon Nanotubes Reveals Changes in Chemokines and Chemokine Receptors. Nanomaterials, 11(4), 883.

Publication 2021

CytoTox96® Infinite® F200 Magellan v7.2 software

Assay Cell viability Cytotox96 Lactate dehydrogenase Promega Radioactive

Corresponding Organization : Karolinska Institutet

Other organizations : IRCCS Humanitas Research Hospital, Center for Research in Molecular Medicine and Chronic Diseases, Universidade de Santiago de Compostela, Chalmers University of Technology, Max Delbrück Center, Southern University of Science and Technology, Freie Universität Berlin, Institute of Occupational Medicine

Top 5 similar protocols

Variable analysis

independent variables

Not explicitly mentioned

dependent variables

Cell viability

control variables

Negative control sample

controls

Negative control sample

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!