Protein Isolation and Western Blot Analysis

Protein isolation and Western blots were performed using standard protocols as described elsewhere^{41 (link)}. The following primary antibodies were used: R2 (Santa Cruz Biotechnology, Dallas, USA, cat. #: sc-10844, 1:1000), Wee1 (Santa Cruz Biotechnology, cat. #: sc-9037, 1:1000), Anti-Histone H3.3 G34W (RevMAb Biosciences, South San Francisco, USA, cat. #: 31-1145-00, 1:1000), Histone H3K36me3 antibody (Active Motif, Cambridge, UK, cat. #: 61021, 1:1000), Cdc2 p34 (Santa Cruz Biotechnology, cat. #: sc-54, 1:500), Phospho-cdc2 Tyrosine 15 (Cell Signaling, Denver, USA, cat. #: #9111, 1:500), and Cyclin B1 (Santa Cruz Biotechnology, cat. #: sc-245, 1:200). As secondary antibody we used: Anti-Rabbit IgG (Sigma-Aldrich, cat. #: A-9169,1:10000), rabbit anti-goat IgG-HRP (Santa Cruz Biotechnology, cat. #: sc-2768, 1:10000) and goat anti-mouse IgG-HRP (Santa Cruz, cat. #: sc-2005, 1:5000). Anti-β-Actin antibody (Sigma-Aldrich, cat. #: A2228, 1:100000) or Anti-β-Tubulin (Sigma-Aldrich, cat. #: T4026,1:2000) were used to detect β-actin or β-tubulin as a housekeeping protein.