Muscle HSP was assessed by Western blotting for muscle HSP25 and HSP72 content using primary polyclonal antibodies specific to muscle HSP25 (ADI-SPP-715, Enzo, Farmingdale, USA) and muscle HSP72 (ADI−SPA−812, Enzo, Farmingdale, USA), diluted 1:1000 in TBST and 2% milk, with secondary antibody diluted 1:20,000 in 3% milk. The overall Western blotting protocol has been previously described [45 (link)].
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