Cytokine Production Quantification in RPE Cells

Cytokine production was measured in the culture supernatants from RPE and RPE^-LC3B cells challenged as described above in the presence or absence of LGM2605 at the concentrations indicated in the Figure legends. Culture supernatants collected at the time points indicated in the figure legends were analyzed by ELISA for IL-1 β (Quantikine Elisa Kit; R&D Systems) and IL-18 (Quantikine Elisa Kit; R&D Systems, #DL180) commercially available kits according to the manufacturer’s instructions [55 (link)]. In each instance, the amount of cytokine present in the supernatant was determined using a standard curve.

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Dhingra A., Sharp R.C., Kim T., Popov A.V., Ying G.S., Pietrofesa R.A., Park K., Christofidou-Solomidou M, & Boesze-Battaglia K. (2021). Assessment of a Small Molecule Synthetic Lignan in Enhancing Oxidative Balance and Decreasing Lipid Accumulation in Human Retinal Pigment Epithelia. International Journal of Molecular Sciences, 22(11), 5764.

Publication 2021

Quantikine Elisa Kit

Cells Cytokine Elisa Il 1 β Il 18 Lgm2605

Corresponding Organization : University of Pennsylvania

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Variable analysis

independent variables

Presence or absence of LGM2605

dependent variables

IL-1β production
IL-18 production

control variables

Challenging of RPE and RPE-LC3B cells

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

Annotations

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