Genomic libraries of low-coverage whole-genome skim sequencing (Malmberg et al. 2018 (link)) were prepared for 24 samples (Supplementary Table S2) from one of the NAM families (Jordan et al. 2018 (link)) using Illumina DNA Prep Kit along with the Illumina’s Nextera CD adapters. Sequencing (2 × 150 bp) was performed on the Illumina NextSeq platform (Kansas State University, Integrated Genomics Facility) for an average of 6.1 million PE reads per accession, which represents ∼0.1× genome coverage.
Jordan K.W., Bradbury P.J., Miller Z.R., Nyine M., He F., Fraser M., Anderson J., Mason E., Katz A., Pearce S., Carter A.H., Prather S., Pumphrey M., Chen J., Cook J., Liu S., Rudd J.C., Wang Z., Chu C., Ibrahim A.M., Turkus J., Olson E., Nagarajan R., Carver B., Yan L., Taagen E., Sorrells M., Ward B., Ren J., Akhunova A., Bai G., Bowden R., Fiedler J., Faris J., Dubcovsky J., Guttieri M., Brown-Guedira G., Buckler E., Jannink J.L, & Akhunov E.D. (2021). Development of the Wheat Practical Haplotype Graph database as a resource for genotyping data storage and genotype imputation. G3: Genes|Genomes|Genetics, 12(2), jkab390.
Corresponding Organization : Kansas State University
Other organizations :
Center for Grain and Animal Health Research, Ithaca College, Cornell University, University of Minnesota, Colorado State University, Washington State University, University of Idaho, Montana State University, Michigan State University, Oklahoma State University, Agricultural Research Service, Edward T. Schafer Agricultural Research Center, University of California, Davis
Genomic libraries of low-coverage whole-genome skim sequencing
dependent variables
Sequencing (2 × 150 bp) was performed on the Illumina NextSeq platform for an average of 6.1 million PE reads per accession, which represents ∼0.1× genome coverage
control variables
Illumina DNA Prep Kit
Illumina's Nextera CD adapters
Kansas State University, Integrated Genomics Facility
controls
No positive or negative controls were explicitly mentioned in the provided information.
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