Animal procedures were performed in accordance with protocol A-2262 approved by the IACUC at the University of Massachusetts Medical School. KRN T cell transgenic mice (provided by Drs. Benoist and Mathis, Harvard Medical School and Institut de Genetique et de Biologie Moleculaire et Cellulaire, Illkirch, France) were crossed with NOD mice (Jackson) to generate arthritic K/BxN mice [24 (link), 25 (link)]. Arthritogenic serum was prepared from 9-week-old K/BxN mice. STA was induced in 12-week-old male IL-17A-deficient mice (Jackson). Absence of IL-17A expression was confirmed by intracellular staining (personal communication, Dr. Keiji Hirota). These mice were backcrossed onto the C57BL/6 background for six generations and C57BL/6J mice (wild-type) were used as controls. Arthritogenic serum, 150 μl per intraperitoneal injection, was administered on days 0, 2, and 7. Ankle thickness was measured as an indicator of inflammation and clinical inflammation was scored as previously described [26 (link)].
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