Inhibition of Inflammatory Pathways

TAK-242 (CAS 243984-11-4 Cat 6587/5) was from R&D Systems (Minneapolis MN). LPS-RS (from Rhodobacter sphaeroides, Catalog # tlrl-rslps) and MCC950 (CAS 210826-40-7, Catalog # inh-mcc) were purchased from InvivoGen (San Diego CA). Other chemicals, including minocycline (CAS 13614-98-7, Cat# M2280000) and IL1-Ra (Cat# SRP 3084), and salts were obtained from Millipore Sigma Chemical Company. Drugs were prepared as stock solutions in either ACSF or DMSO and diluted to final concentration at the time of experiment. The concentrations of TAK-242, LPS-RS and minocycline are based on our previous studies using those inhibitors against LPS²³. The dose and concentrations of MCC950 and IL1-Ra followed studies by Coll et al.^{14 (link)} and Edy et al.^{25 (link)}, respectively.

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Izumi Y., Fujii C., O’Dell K.A, & Zorumski C.F. (2022). Acrylamide inhibits long-term potentiation and learning involving microglia and pro-inflammatory signaling. Scientific Reports, 12, 12429.

Publication 2022

TAK-242 MCC950

Dmso Drugs Inhibitors Mcc950 Minocycline Rhodobacter sphaeroides Salts Tak 242

Corresponding Organization : Washington University in St. Louis

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Variable analysis

independent variables

TAK-242 (CAS 243984-11-4 Cat 6587/5)
LPS-RS (from Rhodobacter sphaeroides, Catalog # tlrl-rslps)
MCC950 (CAS 210826-40-7, Catalog # inh-mcc)
Minocycline (CAS 13614-98-7, Cat# M2280000)
IL1-Ra (Cat# SRP 3084)

dependent variables

Measured outcomes not explicitly mentioned

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