Mitochondrial ATP Production Assay

After the measurement of ADP-stimulated respiration of complex I and complex II, the incubation buffer containing mitochondria was taken from the respiration chamber and immediately supplemented with the ATP assay mix diluted 1:5 (ATP Bioluminescence Assay Kit, Sigma-Aldrich, St. Louis, MO, USA). Mitochondrial ATP production was determined immediately after each respiration measurement and compared with ATP standards using a 96-well white plate in a spectrofluorometer (SpectraMax^® M3, Molecular Devices, San Jose, CA, USA) at 560-nm emission [23 (link),27 (link),28 (link)].

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Rosa F.L., de Souza I.I., Monnerat G., Campos de Carvalho A.C, & Maciel L. (2023). Aging Triggers Mitochondrial Dysfunction in Mice. International Journal of Molecular Sciences, 24(13), 10591.

Publication 2023

ATP Bioluminescence Assay Kit SpectraMax® M3

Assay Bioluminescence assay Buffer Complex i Complex ii Devices Mitochondrial Respiration

Corresponding Organization : National Institute of Science and Technology in Regenerative Medicine

Top 5 similar protocols

Variable analysis

independent variables

ADP-stimulated respiration of complex I
ADP-stimulated respiration of complex II

dependent variables

Mitochondrial ATP production

control variables

Incubation buffer containing mitochondria

controls

ATP standards

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