Neutrophil Depletion Protocol for Muscle Studies

Neutrophil depletion was performed via intraperitoneal administration of 200 µg of anti-Ly6G (1A8, Bio X Cell) antibody 24 h prior to injury induction for the SD-IVM experiment^{12 (link)}. To evaluate neutrophil function in denervated muscle, C57BL/6 mice were injected intraperitoneally with an initial 400 μg followed by 100 μg three times weekly for anti-Ly6G (1A8, Bio X Cell) to deplete neutrophils. Neutrophils were depleted in Ly6g-DTR-GFP mice by intraperitoneal injection of 10 μg/g body weight of diphtheria toxin (DT) three times weekly^{44 (link)}.

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Xiang Y., Dai J., Li Y., You Z., Zhang J., Huang X., Nie S., Chen Y., Xu L., Liu F., Jiang J, & Xu J. (2022). ROS-activated CXCR2+ neutrophils recruited by CXCL1 delay denervated skeletal muscle atrophy and undergo P53-mediated apoptosis. Experimental & Molecular Medicine, 54(7), 1011-1023.

Publication 2022

anti-Ly6G (1A8,

Antibody Body weight C57bl mice Cell Diphtheria toxin Injury Intraperitoneal injection Mice Muscle Neutrophils

Corresponding Organization :

Other organizations : Huashan Hospital, Fudan University, Shanghai Advanced Research Institute, Chinese Academy of Sciences, Shanghai University of Traditional Chinese Medicine, Shanghai Medical College of Fudan University

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Variable analysis

independent variables

Intraperitoneal administration of 200 µg of anti-Ly6G (1A8, Bio X Cell) antibody 24 h prior to injury induction for the SD-IVM experiment
Intraperitoneal injection of an initial 400 μg followed by 100 μg three times weekly for anti-Ly6G (1A8, Bio X Cell) to deplete neutrophils
Intraperitoneal injection of 10 μg/g body weight of diphtheria toxin (DT) three times weekly in Ly6g-DTR-GFP mice to deplete neutrophils

dependent variables

Neutrophil function in denervated muscle

control variables

C57BL/6 mice

positive controls

Not explicitly mentioned

negative controls

Not explicitly mentioned

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