RNA Extraction, qPCR and RNA-seq Protocol

Total RNA was extracted using TRIzol method, and its concentration was tested by NanoPhotometer N120 (IMPLEN, Germany). Real-time PCR was performed as described previously 33 , 34 (link). The primers are listed in the supplementary Table S3. RNA sequencing was performed by BMK (www.biomarker.com.cn). Sequencing libraries were generated using NEBNext UltraTM RNA Library Prep Kit for Illumina (NEB, USA) following the manufacturer's recommendations, and index codes were added to attribute sequences to each sample, sequenced using a NovaSeq 6000 (Illumina) according to the manufacturer's instructions.