Cultivation of Ovarian Cancer Cell Lines

Human ovarian cancer cell line OVCAR5 (female) was obtained from American Type Culture Collection. A2780 (female) was acquired from European Collection of Cell Cultures. OVCA433 (female) ovarian serous adenocarcinoma cell line was a generous gift from Dr. Marcin Iwanicki (Stevens Institute of Technology, Department of Chemistry and Chemical Biology, NJ USA). OVCAR5 and A2780 were grown in RPMI-1640 medium (Corning, catalogue No. 10-040-CM). OVCA433 (Iwanicki et al., 2016)^{39 (link)} were cultured in 1:1 mixture of MCDB 105 (Cell Applications, catalogue No. 117–500) medium and Medium 199 (Gibco, catalogue No. 11150059). All media were supplemented with fetal bovine serum (FBS; Gemini Bio-products, catalogue No. 900–208) and penicillin-streptomycin solution (Corning, catalogue No. 30-002-Cl) at the final concentrations of 10% and 1%, respectively, unless stated otherwise. Cells were kept in a humidified incubator with 5% CO₂ at 37 °C. All cells were tested for mycoplasma infection (MycoAlert Mycoplasma Detection Kit, Lonza, catalogue No. LT07-218) and found to be negative. Cells were counted by means of Bright-Line^™ Hemacytometer (Sigma, catalogue No. Z359629).