Live Imaging of Drosophila Embryogenesis

Flies were raised at 25°C under standard conditions. Pupae were collected for imaging as described previously [52 ]. Ecad::GFP flies [53 (link)] were used for live imaging as previously described [1 (link)]. In brief, images were acquired with a spinning disk microscope from Gataca Systems driven by the MetaMorph software. The system is equipped with an inverted Nikon TI2E stand, a motorized XYZ stage, and a Nikon Plan Apo ×60 oil immersion (NA=1.4) lens and with a Prime95B camera.

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Trébeau C., de Monvel J.B., Altay G., Tinevez J.Y, & Etournay R. (2022). Extracting multiple surfaces from 3D microscopy images in complex biological tissues with the Zellige software tool. BMC Biology, 20, 183.

Publication 2022

TI2E stand Plan Apo ×60 Prime95B camera

Flies Immersion Lens Microscope Pupae

Corresponding Organization : Université Paris Cité

Other organizations : Inserm

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Variable analysis

independent variables

Temperature (25°C)

dependent variables

Pupae morphology/development (for imaging)

control variables

Standard conditions for raising flies

positive controls

Ecad::GFP flies for live imaging

negative controls

No negative controls specified

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