Plasmodium berghei strain NK65 sensitive to chloroquine (CQ),
obtained from Professor O G Ademowo of the Institute of Advanced Medical
Research and Training (IMRAT), University College Hospital, Ibadan, was used to
assess the in vivo chemo-suppressive and curative antimalarial
activity. The parasite strain was preserved via serial passage of blood taken
from an infected mouse into an uninfected mouse. The donor mouse was sacrificed,
and blood was withdrawn through cardiac puncture into a heparinized bottle to
prepare the inoculum. It was diluted with normal saline solution so that 0.2 mL
of the inoculum will contain 1.0 × 107 parasitized red blood
cells.
obtained from Professor O G Ademowo of the Institute of Advanced Medical
Research and Training (IMRAT), University College Hospital, Ibadan, was used to
assess the in vivo chemo-suppressive and curative antimalarial
activity. The parasite strain was preserved via serial passage of blood taken
from an infected mouse into an uninfected mouse. The donor mouse was sacrificed,
and blood was withdrawn through cardiac puncture into a heparinized bottle to
prepare the inoculum. It was diluted with normal saline solution so that 0.2 mL
of the inoculum will contain 1.0 × 107 parasitized red blood
cells.
