Cytokine-Induced Signaling in DC2.4 Cells

DC2.4 cells were cultured in RPMI 1640 with IFN‐γ (10 ng/ml and 100 ng/ml), TNF‐α (10 ng/ml and 100 ng/ml) or LPS (0·5 µg/ml and 5 µg/ml) (all from PeproTech) for 3 h or 12 h at 37° in 5% CO₂. Coimmunoprecipitation and Western blotting were carried out as previously described [45 (link), 46 with antibodies against CD11c, Hsp90, MHCII, Akt/p‐Akt and Erk1/2/p‐Erk1/2 (Cell Signaling Technology).

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Wang Q., Su X., He Y., Wang M., Yang D., Zhang R., Wei J., Ma Q., Zhai W., Pang A., Huang Y., Feng S., Ballantyne C.M., Wu H., Pei X., Feng X., Han M, & Jiang E. (2021). CD11c participates in triggering acute graft‐versus‐host disease during bone marrow transplantation. Immunology, 164(1), 148-160.

Publication 2021

RPMI 1640 IFN‐γ TNF‐α LPS Hsp90 MHCII Akt/p‐Akt

Antibodies Cells Coimmunoprecipitation Erk1 Hsp90 Ifn γ Tnf α

Corresponding Organization : Baylor College of Medicine

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Variable analysis

independent variables

IFN-γ (10 ng/ml and 100 ng/ml)
TNF-α (10 ng/ml and 100 ng/ml)
LPS (0.5 µg/ml and 5 µg/ml)

dependent variables

Protein expression and phosphorylation of CD11c, Hsp90, MHCII, Akt/p-Akt, and Erk1/2/p-Erk1/2

control variables

DC2.4 cells cultured in RPMI 1640 medium
Incubation at 37°C in 5% CO2
Incubation time of 3 h or 12 h

controls

Positive control: Not specified
Negative control: Not specified

Annotations

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