All strains from this study were made by homologous recombination as described (Longtine et al., 1998 (link); Janke et al., 2004 (link); Sheff and Thorn, 2004 (link)). BY4741 and BY4742 yeast strains were purchased from GE Dharmacon (Lafayette, CO). Strains were propagated in rich medium (YPD: 1% yeast extract, 2% peptone, 2% dextrose) or SD minimal medium (0.17% yeast nitrogen base, 0.5% ammonium sulfate, 2% synthetic complete mix, 2% dextrose) supplemented with the appropriate amino acids for plasmid selection. Plasmids were made by homologous recombination in yeast, rescued in Escherichia coli, and confirmed by sequencing. pHPH was made by PCR amplification of the hygromycin resistance gene HPH from pFA6a-hphNT1 and cotransformation of the PCR product with linearized pRS416. Proteins were tagged with either the bright GFP variant GFP+ (Scholz et al., 2000 (link)) or GFP(envy) (Slubowski et al., 2015 (link)), a gift from C. Slubowski (University of Massachusetts, Boston, MA), by PCR amplification of the GFP::HIS cassette and transformation into yeast. Strains and plasmids are described in Supplemental Tables S1 and S2.