Quantification of Neurodegeneration Biomarkers

AcH3K18 ELISA was performed using EpiQuik Global Acetyl Histone H3K18 Quantification Kit (Colorimetric) (EpiGentek). CSF samples were concentrated by 10-fold using Amicon Ultra Centrifugal Filter Unit with Ultracel-3K membrane (Millipore). Mouse β-tubulin ELISA was performed using Mouse TUB β ELISA kit (MyBiosource MBS723313), according to manufacturer’s protocol. Mouse Aβ40 and Aβ42 ELISA was performed using Amyloid β 40 and Amyloid β 42 Mouse ELISA Kit (Thermo Fisher), according to manufacturer’s protocol. Sensitive Tau ELISAs were adapted and modified according to previous reports [24 (link), 25 (link)] and as described previously [41 (link)]. Briefly, mouse monoclonal antibody BT2 or HT7 was used for capture. The respective analytes were detected with alkaline phosphatase–conjugated mouse monoclonal antibody against Tau5 (BioLegend). Recombinant full length hTau (rPeptide) was used to generated standard curves for each assay. The CDP-Star substrate (Invitrogen) was used as chemiluminescent alkaline phosphatase substrate.