Leishmania donovani strain 1S [29 (link)] promastigotes and derived mutants were cultured at 25 °C in M199+ medium [30 (link)] with the respective antibiotics: puromycin (25 µg/mL, AppliChem, Darmstadt, Germany), blasticidin (5 µg/mL), G418 (50 µg/mL) and hygromycin B (50 µg/mL, all Carl Roth, Karlsruhe, Germany). Cells were passaged every 3–4 days.
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