Cell culture supernatants or sera were analyzed by ELISA for IL-1β, TNF, IL-6, IL-12p40, IL-10 (eBiosciences) and Rantes (R&D systems). A sandwich ELISA for mouse IFN-β was performed as described 46 (link). Cytokines from cell culture supernatants were quantitated using the Bio-Rad Bio-PlexTM Mouse Cytokine Assay kit. Transcript abundance of cytokines were determined by quantitative PCR using primers listed in supplementary table S1 .
Protocol detail
Cytokine Quantification in Cell Culture
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Corresponding Organization :
Other organizations : Duke University, University of Massachusetts Chan Medical School, Boston Children's Hospital, Harvard University, Albert Einstein College of Medicine, National Human Genome Research Institute, National Institutes of Health, University of California, San Diego, University of Gothenburg
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Variable analysis
independent variables
- Cell culture supernatants or sera
- IL-1β
- IL-12p40
- IL-10
- Rantes
- IFN-β
- Cytokines from cell culture supernatants
- Not explicitly mentioned
- Positive control: None mentioned
- Negative control: None mentioned
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