Cultivation of Human Glioblastoma and Cell Lines

Human glioblastoma multiforme T98G (ATCC, CRL-1690), U87MG (ATCC, HTB-14) and KJT23I (patient-derived) cells as well as human embryonic kidney HEK293T cells (ATCC, CRL-3216), primary human bronchial fibroblasts NHLF and mouse macrophages MAC (ATCC, CCL-46) were routinely cultivated in standard conditions with DMEM medium supplemented with 10% fetal bovine serum (FBS) and 1% antibiotics: penicillin/streptomycin cocktail as it was described elsewhere^{33 (link)}. For each experiment, cells were harvested with 0.25% Trypsin–EDTA solution (Gibco), counted in a Z2 particle counter (Beckman Coulter), and seeded at an appropriate density into tissue culture plates (Eppendorf).

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Janik-Olchawa N., Drozdz A., Ryszawy D., Pudelek M., Planeta K., Setkowicz Z., Sniegocki M., Wytrwal-Sarna M., Gajewska M, & Chwiej J. (2021). The influence of IONPs core size on their biocompatibility and activity in in vitro cellular models. Scientific Reports, 11, 21808.

Publication 2021

Trypsin–EDTA solution Z2 particle counter tissue culture plates

Antibiotics Cells Edta Embryonic Fetal bovine serum Fibroblasts Glioblastoma multiforme Human Kidney Macrophages Mouse Patient Penicillin Primary bronchial Streptomycin Tissue Trypsin

Corresponding Organization :

Other organizations : AGH University of Krakow, Jagiellonian University, Nicolaus Copernicus University

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Variable analysis

independent variables

Cell type (human glioblastoma multiforme T98G, U87MG, KJT23I; human embryonic kidney HEK293T; primary human bronchial fibroblasts NHLF; mouse macrophages MAC)

dependent variables

Cell growth/proliferation (not explicitly stated, but implied by the mention of 'seeding cells at an appropriate density')

control variables

Culture media (DMEM with 10% FBS and 1% antibiotics)
Cell culture conditions (standard)
Cell harvesting method (0.25% Trypsin-EDTA solution)

controls

No positive or negative controls were explicitly mentioned in the provided information.

Annotations

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