Sorafenib and HDAC Inhibitor Combination Therapy

Sorafenib (purity ≥ 99%) was purchased from Biovision. MPT0E028 and vorinostat (purity ≥ 98%) were synthesized by Dr. Jing-Ping Liou's Laboratory (Taipei Medical University, Taipei, Taiwan; ref. 13 (link)). EGTA, EDTA (disodium salt), leupeptin, dithiothreitol, propidium iodide, MTT, phenylmethylsulfonylfluoride (PMSF), ribonuclease A, z-VAD– FMK, Ac-DEVD–CHO, PD98059, PD173074, and all of the other chemical reagents were obtained from Sigma. RPMI-1640 medium, Dulbecco's Modified Eagle Medium (DMEM), FBS, penicillin, streptomycin, and all other tissue culture regents were obtained from GIBCO/BRL Life Technologies. The following antibodies were used: caspase-8, caspase-9, p21, histone H3, acetyl-a-tubulin, phospho-Erk, and Erk (Cell Signaling Technology); Mcl-1, PARP, and FGFR3 (Santa Cruz Biotechnology); acetyl-histone H3 and pan-actin (Millipore); caspase-3 (Imgenex); phospho-Stat3- Tyr⁷⁰⁵ and phospho-Stat3-Ser⁷²⁷ (Epitomics); and Stat3 (BD Biosciences).

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Chen C.H., Chen M.C., Wang J.C., Tsai A.C., Chen C.S., Liou J.P., Pan S.L, & Teng C.M. (2014). Synergistic Interaction between the HDAC Inhibitor, MPT0E028, and Sorafenib in Liver Cancer Cells In Vitro and In Vivo. Clinical cancer research : an official journal of the American Association for Cancer Research, 20(5), 1274-1287.

Publication 2014

Ac devd cho Actin Antibodies Caspase 3 Caspase 8 Caspase 9 Dithiothreitol Eagle Edta Egta Fgfr3 Histone h3 Leupeptin Mpt0e028 Pd173074 Pd98059 Penicillin Propidium iodide Ribonuclease Salt Sorafenib Stat3 Streptomycin Tissue Tubulin Vorinostat Z vad fmk

Corresponding Organization : The Ohio State University

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Variable analysis

independent variables

Sorafenib (purity ≥ 99%)
MPT0E028
Vorinostat (purity ≥ 98%)

dependent variables

Not explicitly mentioned

control variables

EDTA (disodium salt)
Leupeptin
Dithiothreitol
Propidium iodide
Phenylmethylsulfonylfluoride (PMSF)
Ribonuclease A
Z-VAD–FMK
Ac-DEVD–CHO
PD98059
PD173074
RPMI-1640 medium
Dulbecco's Modified Eagle Medium (DMEM)
Penicillin
Streptomycin

positive controls

Not specified

negative controls

Not specified

Annotations

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