Protocol detail

ChIP-seq of Pseudomonas aeruginosa regulator

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ChIP was performed as previously [81 (link),82 (link)]. P. aeruginosa ΔPA2577 strain carrying pMEB12 (araBADp-PA2577-flag) or pKGB8 vectors was grown in a medium containing 0.02% arabinose as the inducer. Additionally, it was used in the ChIP procedure with anti-flag antibodies (MA1-91878, Invitrogen). Data were processed essentially as previously [82 (link)]. Sequencing data are available in the NCBI’s Gene Expression Omnibus (GEO) database (http://www.ncbi.nlm.nih.gov/geo/ (accessed on 30 October 2021)), under accession number GSE186746.

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Modrzejewska M., Kawalek A, & Bartosik A.A. (2021). The Lrp/AsnC-Type Regulator PA2577 Controls the EamA-like Transporter Gene PA2576 in Pseudomonas aeruginosa. International Journal of Molecular Sciences, 22(24), 13340.

Publication 2021

MA1-91878

Anti antibodies Arabinose Chip P aeruginosa Strain Vectors

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Variable analysis

independent variables

Strain (Δ PA2577 strain carrying pMEB12 (araBAD p-PA2577-flag) or pKGB8 vectors)

dependent variables

Genomic regions bound by PA2577-flag protein (as determined by ChIP-seq)

control variables

Growth medium containing 0.02% arabinose as the inducer
Anti-flag antibodies (MA1-91878, Invitrogen) used in the ChIP procedure

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

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