Infrared Spectroscopy of Cactus Fruit Extracts

The infrared spectroscopy analyses of Epiphyllum, Hylocereus, and Opuntia fruits’ dry methanolic extracts were performed on a Nicolet FT-IR 6700 spectrometer (Thermo Electron Corporation, Cambridge, UK) with Omnic™ Spectra Software (Thermo Scientific, Waltham, MA, USA), in attenuated total reflectance sampling (ATR) mode. The infrared spectrum was measured in the mid-infrared region (4000–600 cm⁻¹). The position and intensity of the absorption bands in the FTIR spectra were used to analyse the functional groups according to libraries and bibliography [30 (link),31 ,32 ,33 (link)].

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Barkociová M., Tóth J., Sutor K., Drobnicka N., Wybraniec S., Dudík B., Bilková A, & Czigle S. (2021). Betalains in Edible Fruits of Three Cactaceae Taxa—Epiphyllum, Hylocereus, and Opuntia—Their LC-MS/MS and FTIR Identification and Biological Activities Evaluation. Plants, 10(12), 2669.

Publication 2021

Nicolet FT-IR 6700 spectrometer Omnic™ Spectra Software

Electron Fruits Ftir Methanolic Opuntia Spectroscopy

Corresponding Organization : Comenius University Bratislava

Other organizations : Cracow University of Technology

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Variable analysis

independent variables

Type of cactus fruit (Epiphyllum, Hylocereus, Opuntia)

dependent variables

Functional groups in the dry methanolic extracts of the cactus fruits as determined by infrared spectroscopy

control variables

Nicolet FT-IR 6700 spectrometer (Thermo Electron Corporation, Cambridge, UK) with Omnic™ Spectra Software (Thermo Scientific, Waltham, MA, USA)
Attenuated total reflectance (ATR) sampling mode
Mid-infrared region (4000–600 cm^-1) for infrared spectrum measurement

controls

Positive and negative controls not explicitly mentioned

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