Neurophysiological and EMG data were collected using a data acquisition system (Tucker-Davis Technologies, Gainsville, FL) with sampling rates of approximately 25 and 1.5 kHz, respectively. Neural data were collected simultaneously from two cortical locations using 16-site, 3 or 5 mm silicon-iridium electrodes (A1x16-3 mm-100-177-A16 or A1x16-5 mm-100-177-A16; NeuroNexus, Ann Arbor, MI). Before insertion, electrodes were coated with a fluorescent dye (DiI; Invitrogen, Waltham, MA) for later confirmation of placement. A chlorinated silver wire (0.25 mm in diameter; Medwire, Mt. Vernon, NY) was inserted into occipital cortex and used as both reference and ground. Neural data were recorded and visualized using Synapse software (Tucker-Davis Technologies). Video was collected using a BlackFly-S camera (100 fps) and SpinView software (FLIR Integrated Systems, Wilsonville, OR). To enable synchronization of the video and electrophysiological records, an LED was positioned within the camera frame and was programmed to flash once every 3 s (Dooley et al., 2021 (link)).
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