Maine Coon Cat Blood DNA Extraction

Fifty-five Maine Coon cats were recruited into this study. The procedures of animal use were permitted by Kasetsart University Institutional Animal Care and Use Committee, Kasetsart University, Bangkok, Thailand (Approval number: ACKU 62-VET-059). The blood samples were collected from venous vessels for 2–3 ml per cat and stored in an ethylenediaminetetraacetic acid (EDTA) tube. The stored blood was aliquoted and kept at −20°C until DNA extraction. As described previously, the process of DNA extraction was conducted via Blood Genomic DNA Extraction Mini Kit (Favorgen, Taiwan) regarding manufacturer's recommendations (23 (link)).

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Sukumolanan P., Demeekul K, & Petchdee S. (2022). Development of a Loop-Mediated Isothermal Amplification Assay Coupled With a Lateral Flow Dipstick Test for Detection of Myosin Binding Protein C3 A31P Mutation in Maine Coon Cats. Frontiers in Veterinary Science, 9, 819694.

Publication 2022

Blood Genomic DNA Extraction Mini Kit

Animal Blood Cat 2 Cats Ethylenediaminetetraacetic acid Genomic Institutional animal care and use committee Venous vessels

Corresponding Organization :

Other organizations : Kasetsart University, Naresuan University

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

None explicitly mentioned

control variables

Blood sample collection volume (2-3 ml per cat)
Blood sample storage (in EDTA tube at -20°C)
DNA extraction process (using Blood Genomic DNA Extraction Mini Kit)

positive controls

None explicitly mentioned

negative controls

None explicitly mentioned

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