The experiment was implemented with the assistance of the RIP™ RNA-Binding Protein Immunoprecipitation Kit (Merck Millipore). RIP lysis buffer was administered to lyse LoVo and LS513 cells, and RNA (miR-NC or miR-195-5p) magnetic beads were combined with the mouse anti-IgG antibody and the human anti-Ago2 antibody or human anti-IGF2BP2 antibody [34 (link)]. TUG1’s level was determined through qRT-PCR.
Free full text: Click here